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應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結(jié)論、致謝!

來源:上海謂載 瀏覽 1353 次 發(fā)布時間:2021-10-20


結(jié)論


首先,表面張力測量證明牛精漿具有非常好的表面活性,這可能與 BSP 蛋白。 二、朗繆爾薄膜法 先前被證明是相關(guān)的脂質(zhì)單層模型 公牛精子的外細胞膜 [35],有助于表征 BSP 蛋白對脂質(zhì)膜的親和力。 BSP 蛋白是 能夠到達被磷脂覆蓋的牛精子細胞表面,這要歸功于它們與磷脂酰膽堿和 它們自身的表面活性。 我們的假設(shè)是,由于它們自己的表面,它們首先穿透精子的外葉 活動然后他們留在那里因為與 磷脂酰膽堿。 最后,Langmuir 薄膜法也得到了 用于篩選已知的 BSP 蛋白螯合劑(如 LDL 和脂質(zhì)體)的作用。 脂質(zhì)體被證明是 與 LDL 一樣有效地防止 BSP 蛋白插入 磷脂層。 發(fā)現(xiàn) LDL 和 脂質(zhì)體:0.16–0.17 mg 磷脂酰膽堿/mg BSP。 我們不 還知道脂質(zhì)體在 冷凍保存的時間尺度有一些生物學后果。 需要進一步的研究來分析每個的表面特性 BSP 蛋白及其對膜的作用。 此外,可以測試朗繆爾薄膜以檢測 BSP 對膜的親和力隨溫度的變化 [20]。 最后,朗繆爾薄膜法是一種 用于鑒定新的螯合劑的強大篩選方法 BSP 蛋白質(zhì)。 這種方法可以適用于其他動物物種。


致謝


作者要感謝 IMV Technologies(法國) 其財政支持,Gérard Chatagnon 的量化 精漿中的蛋白質(zhì),凝膠電泳的 Véronique Solé,英語校正的 Maureen Collobert 和 Alain Sire 和 Patrice Papineau 的概念和實現(xiàn) 手套箱允許在受控的情況下使用 Langmuir 槽進行工作 大氣層。


附錄 A. 補充數(shù)據(jù)


與本文相關(guān)的補充數(shù)據(jù)可以在 在線版本,見 http://dx.doi.org/10.1016/j.colsurfb.2015.11。 034.


參考


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應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——摘要、簡介

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——材料與方法

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結(jié)果與討論

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結(jié)論、致謝!

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